Molecular Expressions Cell Biology Mitosis with Fluorescence Biology Diagrams The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice
Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator)is a set of fluorescent probes which enable the visualization of cell cycle progression in living cells. Fucci takes advantage of the fact that the replication licensing factors Cdt1 and Geminin are only present during specific phases of the cell cycle. A fusion protein of a fragment Fucci (CA) yields abundant expression of mCherry throughout G1 phase with an abrupt decrease in the red fluorescence at the end of G1 phase. Fucci (CA) can be used to reliably detect a short G1 phase and distinguish S and G2 phases, which previously was difficult to achieve. (CA) Figure1: Visualization of Cell-Cycle Progression by Fucci (SA
Visualizing Spatiotemporal Dynamics of Multicellular Cell Biology Diagrams
Abstract. Proper cell-cycle progression is essential for the self-renewal and differentiation of human pluripotent stem cells (hPSCs). The fluorescent ubiquitination-based cell-cycle indicator (FUCCI) has allowed the dual-color visualization of the G 1 and S/G 2 /M phases in various dynamic models, but its application in hPSCs is not widely reported. In addition, lineage-specific FUCCI By visualizing the cell cycle, Fucci is a powerful tool to investigate any process that has to do with cell growth and differentiation, such as the development and regeneration of organs as well as carcinogenesis. Cells with pure green fluorescence were either in the S or G2 phase and were distinguished by immunostaining of the S phase. The
A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and Similarly to FUCCI, these proteins carry cell cycle-dependent degradation motifs to resolve G1 and S/G2/M phases. We showed utility of FUCCI-Red by visualizing cell cycle progression of cancer cells in 2D and 3D cultures and monitoring development of tumors in vivo by confocal and fluorescence lifetime imaging microscopy (FLIM).
color cell cycle indicator for fluorescence ... Biology Diagrams
The fluorescence ubiquitination cell-cycle indicator (FUCCI) was developed in 2008 by Miyawaki et al., which color-codes the phases of the cell cycle in real-time. Therefore, visualizing cell cycle dynamics within tumors with FUCCI can provide a guide for many strategies to improve cell-cycle targeting therapy for solid cancers. Keywords:
The widely used fluorescence ubiquitination cell cycle indicator (FUCCI) system, based on the expression of the cell cycle oscillators Cdt1 and geminin tagged with different fluorescent proteins
